CD32(+)CD4(+) memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice.

  • HIV Clinical and Translational Research
  • Platform National Cytometry
January 22, 2021 By:
  • Adams P
  • Fievez V
  • Schober R
  • Amand M
  • Iserentant G
  • Rutsaert S
  • Dessilly G
  • Vanham G
  • Hedin F
  • Cosma A
  • Moutschen M
  • Vandekerckhove L
  • Seguin-Devaux C.

CD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4(+) T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32(+) memory CD4(+) T cells were enriched in cell-associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32(-)CD4(+) fraction. Using multidimensional reduction analysis, several memory CD4(+)CD32(+) T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32(+)CD4(+) memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32(-)CD4(+) T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4(+) T-cell subsets that contain only a small proportion of the translation-competent reservoir.

2021 Jan. iScience.24(1):101881.
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